FIG. 2.

In vitro transcription of the wt RNA-NP with the charge-to-alanine mutant P proteins. (A and B) VVT7-infected A549 cells transfected with no plasmids (−) or cotransfected with wt or mutant P plasmids together with L plasmid. Cytoplasmic cell extracts were incubated with wt RNA-NP in the presence of [α-³²P]CTP. The transcripts were purified and analyzed by gel electrophoresis as described in Materials and Methods. The positions of the NP and P transcripts are indicated. (C and D) Immunoblot analysis of samples of the cytoplasmic extracts with α-P antibody, where the position of the P protein is indicated. Panels C and D correspond to the samples in panels A and B, respectively.