FIG. 5.

Cobinding of the charge-to-alanine mutant P proteins and the GST-P fusion protein to glutathione-Sepharose beads. (A to C) VVT7-infected A549 cells were transfected with the wt or mutant P plasmids alone or together with GST-P plasmid as indicated. The cells were labeled with Tran³⁵S-label, and cell extracts prepared and incubated with glutathione-Sepharose beads; the bound proteins were then analyzed by SDS-PAGE as described in Materials and Methods. The positions of the P and GST-P proteins are indicated.