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. 2024 Jul 9;13(7):512. doi: 10.3390/biology13070512

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Imputation of the adult mouse visual cortex using MAGIC and sc-PHENIX. (A) Recovered Flt1 expression by MAGIC and sc-PHENIX are visualized on UMAP projection of the adult mouse visual cortex cells dataset with different parameter combinations. (B) The non-imputed expression values of Flt1 are visualized on UMAP projection of the adult mouse visual cortex cells dataset. (C) In the 2D UMAP projection of the adult mouse visual cortex cells dataset (without imputation), three main clusters are GABAergic (green circle), glutamatergic (orange circle), and non-neuronal cell types, with 21 cell phenotypes in total. Different parameters were used to see the effect of over-smoothing for MAGIC and sc-PHENIX methods.