Figure 3.

Representative Western blots and relative quantification of (a) GAP43, (b) synaptophysin, (c) pPSD-95-Ser295, total PSD, and their ratio, (d) pCAMKIIα-Thr286, total CAMKIIα, and their ratio, in the hippocampi of the prenatally Dx-treated and control rats, drinking water or fructose after weaning. β-actin and GAPDH were used for the normalization of immune-positive bands of target proteins. The expression of the target proteins in each experimental group was determined as the fold change relative to the appropriate controls that were assigned the value 1. The results are shown as mean ± SEM (n = 6 animals per group). Effects of fructose consumption and Dx treatment were determined by two-way ANOVA. A value of p < 0.05 was considered statistically significant. Significant between-group differences from post hoc Tukey test are given as * p < 0.05.