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. 2024 Jul 19;13(7):864. doi: 10.3390/antiox13070864

Figure 2.

Figure 2

Effect of DOX and CD on the mitochondrial membrane potential (Δψm) in A375, H9C2 and NHDF. (A–C): A375 (A), H9C2 (B) and NHDF (C) were treated with DOX and CD alone or in combination for 2 or 4 h, followed by tetramethylrhodamine methyl ester (TMRM, 100 nM) staining. CCCP served as positive control. The level of TMRM was determined by flow cytometry using CyFlow Cube 6, and FlowJo software was used to calculate the median fluorescent intensity (MFI). Mock-treated controls were set to 100%. Data represent means ± SEM, n ≥ 3. One-way ANOVA was used to determine statistical significance compared to respective control. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.