Figure 3.

(A) Schematic representation of hippocampus isolation, enzymatic treatment, and culture. (B) Microphotography of cultured hippocampal cells (scale bar 10 μm, magnification ×200). (C) schematic representation of in vitro treatment protocol of cells with imiquimod, cinnamon followed by imiquimod, or the plasma of different mice groups. (D) 2′,7′-dichlorofluorescein (DCF) fluorescence variation after imiquimod or plasma treatment of hippocampal neurons. (E,F) Difference in fluorescence between groups at baseline (before imiquimod treatment) and after imiquimod treatment. (G) Difference in fluorescence between groups after treatment of hippocampal cells with the plasma of different mice groups. Cinna: cinnamon. Imq: imiquimod. a.u.: arbitrary units. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.