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. 2024 Jul 9;16(14):2500. doi: 10.3390/cancers16142500

Figure 5.

Figure 5

Reduced secretion of exosomes alters macrophage phenotypes: (a) Rab27a siRNAs are transfected into the GL261-GSC cells and then the expression of rab27a is assessed by qRT-PCR analysis. GAPDH is used as a reference gene (n = 3); (b) the expression of Rab27a and exosomes marker TSG101 was detected by Western blot; (c) relative quantitative results of the Griess experiment of in the culture medium of BMDMs cultured with the culture medium of si-NC or siRab27a-GL261-GSC cells (n = 3); (d) relative quantitative results of ELISA experiment of TGF-β and IL10 protein levels in the culture medium of BMDMs cultured with the culture medium of si-NC or siRab27a-GL261-GSC cells (n = 3); (e) relative quantitative results of qRT-PCR analysis of the expression IL-10 and Arg-1 in BMDMs cultured with the culture medium of si-NC or siRab27a-GL261-GSC cells. GAPDH is used as a reference gene (n = 3). Data are presented as the mean ± S.D. p-value is determined by Student’s t-test or ordinary one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001).