Table 3.
List of primary antibodies.
| Antibody Specificity | Source, Host Species | Dilution (in TBST or PBST) |
|---|---|---|
| Anti-Bax | Santa Cruz Biotechnology (Dallas, Texas, USA), mouse monoclonal | 1:500 |
| Anti-Bcl-2 | Santa Cruz Biotechnology (USA), rabbit polyclonal | 1:500 |
| Anti-Casp3 | Santa Cruz Biotechnology (USA), rabbit polyclonal | 1:500 |
| Anti-phospho-SAPK/JNK (Thr183/Tyr185) | Cell Signaling Technology (Danvers, Massachusetts, USA), rabbit polyclonal | 1:500 |
| Anti-JNK | Santa Cruz Biotechnology (USA), rabbit polyclonal | 1:500 |
| Anti-phospho-Akt (Ser473) | Cell Signaling Technology (USA), rabbit polyclonal | 1:1000 |
| Anti-total Akt | Cell Signaling Technology (USA), rabbit polyclonal | 1:1000 |
| Anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling Technology (USA), rabbit polyclonal | 1:1000 |
| Anti-p44/42 MAPK (Erk1/2) | Cell Signaling Technology (USA), rabbit polyclonal | 1:1000 |
| Anti- NF-kB p65 | Cell Signaling Technology (USA), rabbit polyclonal | 1:2000 |
| Anti-BDNF | Santa Cruz Biotechnology (USA), rabbit polyclonal | 1:500 |
| Anti- NMDA Receptor 1 (GluN1) | Cell Signaling Technology (USA), rabbit monoclonal | 1:1000 |
| Anti- NMDA Receptor 2A (GluN2A) | Merck Millipore (Burlington, Massachusetts, USA), rabbit monoclonal | 1:1000 |
| Anti-NMDA Receptor 2B | Abcam (Cambridge, UK), mouse polyclonal | 1:4000 |
| Anti-EAAT2 (GLT-1) | Abcam (UK), rabbit monoclonal | 1:1000 |
| Anti-PSD95, clone 7E3-1B8 | Merck Millipore (USA), mouse monoclonal | 1:2000 |
| Anti-β-actin | Abcam (UK), mouse monoclonal- conjugated to HRP | 1:10,000 |
| Anti-β-tubulin | Santa Cruz Biotechnology (USA), mouse monoclonal | 1:1000 |