Skip to main content
. 2024 Jun 27;12(7):1439. doi: 10.3390/biomedicines12071439

Figure 7.

Figure 7

Detection of apoptosis rate using Annexin V-FITC/Propidium Iodide (PI) in human corneal endothelial cells (HCECs) after transfection with Accell Control-siRNA or Bax+Bak-siRNA compared to untransfected cells (Cells only). After incubation for 96 h, cells were incubated with either apoptosis inducers (a) staurosporine (250 nM for 6 h) or (b) etoposide (42.5 µM for 21 h). Representative flow cytometric analysis of HCECs under the respective conditions. (FACSCalibur, BD; cells in Q1: necrosis, Q2: late apoptosis, Q3: early apoptosis; Q4: live; FL-1: Annexin, FL-2: PI). Quantification shows that significantly more live cells were present after the Bax+Bak knockdown. (FlowJo; n = 3, mean ± s.e.m., RM one-way ANOVA posthoc uncorrected Fisher’s LSD test: * p < 0.05).