Figure 4.

rpmb2 does not augment BCG-mediated protection against Mtb. (A–C) An incremental increase in the secretion of IL–2 upon incubation of rpmb2-specific and Ag85B-specific T-cell hybridomas with H37Rv-infected bone-marrow-derived dendritic cells (BMDC) was noted. IL–2 secretion increased with an increase in the MOI of H37Rv infection and was positive upon incubation with rpmb2 (B,C). Ag85B–specific T-cell hybridomas were used as a positive control and did not stimulate IL–2 secretion upon incubation with rpmb2 (A). (D–F) BCG–vaccinated mice were boosted with rmpb2, but this did not lead to an increment in the protection mediated by BCG, as illustrated by spleen, lung, and lymph node CFUs (D–F). ** for p < 0.001, *** for p < 0.0001, **** for p < 0.00001 by ANOVA.