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. 2024 Jul 18;15(7):940. doi: 10.3390/genes15070940

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Synonymous codon usage and selective pressures in the evolution. (A): Codon content of 20 amino acids and stop codons in all protein-coding genes of four S. japonicus chloroplast genome; (B): Distribution of codon preference in S. japonicus; (C): Ka/Ks values of protein-coding genes of the five comparative combinations. Note: in (A), the top panel shows the RSCU for the corresponding amino acids, and the colored blocks, which are shown below, represent different codons. In (C), Ka: nonsynonymous; Ks: synonymous.