Figure 2. Spine-specific analysis of STORM imaging of synaptic protein PSD-95.
(A) STORM reconstruction of PSD-95 (increasing intensity color-coded from purple to bright orange) overlaid on diffraction-limited YFP image (yellow). Two example ROIs contain a single spine.
(B) Nanomodules of PSD-95. The same two ROIs and their scatterplots of SMLM localizations.
(C) Nanodomains of PSD-95. The same two ROIs and their scatterplots of SMLM localizations.
(D) Nanomodules of PSD-95 assigned to individual spines (magenta) or dendrites (cyan).
