Fig. 1: Slc30a10−/− mice have increased Mn absorption.
(a) Two-month-old female (F) and male (M) Slc30a10+/+ (‘+’) and Slc30a10−/− (‘-’) mice were analyzed for Mn absorption by 54Mn gavage. (b-d) P14–28 Slc30a10+/+ and Slc30a10−/− mice were analyzed for Mn absorption (b), liver Mn levels by ICP-OES (c), and RBC counts by complete blood counts (d). (e, f) Wild-type mice weaned onto diets containing 1, 100, or 2400 ppm Mn were analyzed at two months of age for liver Mn levels (e) and 52Mn absorption (f). (g) X-ray fluorescence microscopy of small intestinal sections from two-month-old Slc30a10+/+ and Slc30a10−/− mice. (h) Immunofluorescence for Dmt1 (h) and Fpn (i) in small intestinal sections from two-month-old Slc30a10+/+ and Slc30a10−/− mice. Apical Dmt1 and basolateral Fpn staining are indicated by arrowheads. For (a-f), data are represented as means +/− standard deviation, with at least three animals per group. Data were tested for normal distribution by Shapiro-Wilk test; if not normally distributed, data were log transformed. Within each sex, groups were compared using unpaired, two-tailed tests (a-d) or one-way (e, f) ANOVA with Tukey’s multiple comparisons test. (* P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001)