Extended Data Fig. 5. Slide-seq data demonstrating changes in cortical interneurons in Fezf2 mutants.

a, Representative Slide-seq data on a single coronal brain section of the SSp cortex from (left) a P37 WT mouse and (right) a P37 Fezf2 KO mouse. b, Stacked Slide-seq data aligning multiple brain sections of P28–37 WT mice, illustrating the distribution of PNs. MO: n=4 ROIs, n=3 mice; SSp: n=8 ROIs, n=5 mice; VISp: n=4 ROIs, n=4 mice. c, Stacked Slide-seq data from 4–6 weeks old Fezf2 KO mice. MO: n=3 ROIs, n=3 mice; SSp: n=6 ROIs, n=5 mice; VISp: n=4 ROIs, n=2 mice. d-e, Deep-layer PVALB and SST interneurons identified in control and Fezf2 KO mutants (same dataset as in b-c), highlighting selective PVALB and SST interneuron subtypes. f, Ridge plots showing the distribution of selected PVALB and SST subtypes in control and Fezf2 KO brains, demonstrating the shifted laminar location of PVALB-Fzd6 and SST-Chrna2 towards L6 in Fezf2 mutants. The same dataset is used as in Fig. 2g.