Figure 6:

PACCs have a pEMT phenotype: A) RNA expression of a panel of EMT markers by RTqPCR in a PC3-Luc parental population vs. size filtered PACC population. B) Protein expression a panel of EMT markers by Western blot in a PC3-Luc parental population vs. size filtered PACC population, where each biological replicate reported is an average of three technical replicates. C) Summary table of RNA and protein expression. D) Representative immunofluorescent mages of PACCs identified as DTCs in the bone marrow of a metastatic prostate cancer patient, stained for DAPI, an Epithelial-origin cocktail, and VIM. CNV analysis of the corresponding single cell.