Figure 4. Autophagy deficiency in myeloid cells promotes the growth of genetically induced tumors while suppresses the growth of implanted tumors.

(A-D) Mammary tumors were genetically induced in MMTV-PyMT + Atg5^flox/flox (MP-WT) and MMTV-PyMT + Atg5^flox/flox + LysMcre (MP-KO) mice. Total mammary tumor mass per mouse was measured from (A) 65 day, (B) 80 day, and (C) 95 day old mice. (D) Each data point represents the average number of metastatic foci counted from 6 sections per lung. (E) 1×10⁵ tumor cells from MMTV-PyMT mice were subcutaneously implanted onto Atg5^flox/flox (Atg5 WT) and Atg5^flox/flox + LysMcre (Atg5 KO) female mice. Tumor volumes on the left were measured every 2 days. At the end point, tumors were collected, and the weights were measured as shown on the right. Total number of mice used for each study (n) are indicated in each figure, and each data point in tumor mass represents each mouse. The p values for tumor volumes were calculated with two-way ANOVA and the statistical significance for tumor mass and lung metastasis were calculated with Mann-Whitney test. *p < 0.05, **p < 0.01, ***p <0.001, n.s.: not significant.