Figure 5. Impact of autophagy deficiency in myeloid cells on immune cell recruitment in genetically induced tumors.

Tumors from 65-day-old mice, MMTV-PyMT + Atg5^flox/flox (MP-WT) and MMTV-PyMT + Atg5^flox/flox + LysMcre (MP-KO), were collected, and infiltrated immune cells were analyzed. (A) Percentage of immune cells within the live cell population in tumor tissue. (B-E) Percentage of (B) myeloid cell (C) tumor associated macrophages, CD11b⁺ Ly6G⁻ Ly6C⁻, (D) polymorphonuclear MDSCs, CD11b⁺ Ly6G^high Ly6C^low, (E) monocytic MDSCs, CD11b⁺ Ly6G⁻ Ly6C^high within CD45+ population in tumor tissue. (F,G,I) Percentage of (F) CD8⁺ T cells, CD3⁺ CD8⁺, (G) CD4⁺ T cells, CD3⁺ CD4⁺, (I) regulatory T cells, CD3⁺ CD4⁺ CD25⁺ Foxp3+ within CD45+ population in tumor tissue. (H) The ratio of CD8⁺ T cells to CD4⁺ T cells. Total number of mice used for each study (n) are indicated in each figure, and each data point represents each mouse. Error bars indicate mean +/− SEM, and statistical significance was calculated with Mann-Whitney test. *p < 0.05, **p < 0.01, n.s.: not significant.