Figure 6.

Processing pipeline for HiP-CT imaging and segmentation of human kidney vasculature. (A) Setup for imaging acquisition using HiP-CT at BM05; 1) tomographic stage, 2) platform, 3) sample, 4) reference sample (B) Image processing pipeline; 1) a 2D reconstructed image at 25 μm3 /voxel resolution; 2) binning the image by 2, 3) applying 3D median filter to increase signal-to-noise ratio, 4) Image normalization using background detection correction, 5) Segmentation and thresholding, 6) Labelling the four main arterial branches (C) 3D rendering of the segmented vascular network of a human kidney. Each of the main four branching of the renal artery entering the kidney are colour-coded, Figure after²⁰