FIG. 3.

Northern blot analysis of RNA isolated from MCMV-infected MEF at immediate-early (lanes 1), early (lanes 2), and late (lanes 3) times p.i. RNA from uninfected MEF was included as a control. Total RNA (5 μg) was separated in a denaturing agarose gel, blotted onto nylon, and hybridized with double-stranded DNA probes. The specificity of the DNA probes is indicated in Fig. 1A. Solid arrows denote the 1-kb m131/129 transcript in wt MCMV and in Δm131ns.