FIG. 6.

Immunoblotting of MHV virion proteins by structural and gene 1 protein antibodies. MHV virions were purified by sucrose gradient centrifugation. Virions were lysed in SDS buffer, separated on an SDS–15% acrylamide gel, transferred to polyvinylidene difluoride, and incubated with antibodies directed against the proteins as indicated above the lanes. Equivalent amounts of virion proteins were present in each lane. The anti-M and anti-N mouse MAbs J.1.3 and J.3.3, obtained from John Fleming, were used as primary antibodies at a 1:1,000 dilution. The rabbit polyclonal antibodies directed against 3CLpro (SP9) and Hel (B1) were used as primary antibodies at a 1:100 dilution.