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. 2024 Jul 21;25(14):7976. doi: 10.3390/ijms25147976

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reactivity of antibody preparations from chickens immunized with either the RBD antigen (SARS-CoV-2 receptor-binding-domain coupled to LS-particles), the S1 antigen (S1 subunit of the SARS-CoV-2 spike protein coupled to LS-particles) or an inactivated SARS-CoV-2 full-virus preparation tested in immunofluorescence assay. (A) Serum and (B) yolk preparations from chickens are reactive with RBD antigen on immunofluorescence plates showing fluorescence signal of antibody preparations raised against recombinant RBD, S1 or whole-virus preparations. Bar 20 µm. (C) Yolk from the RBD group was also positive against the Delta and Omicron BA.1 variant. Bar 100 µm.