Table 1.

Details of the GEO dataset study designs used to examine BCKDK gene expression in different models.

S. No	GEO Database Identification No.	Treatment	Study Design	Model
1	3242858	Age compared	Needle biopsies of the vastus lateralis of the left leg were collected from seven 20–29 yo females and eight 65–71 yo females. RNA was extracted from the muscle and reverse transcribed to cDNA with oligo-dT-[T7] as the primer. The cDNA was then converted to cRNA and probed using Affymetrix (Santa Clara, CA, USA) HG-U133A and HF-U133B high-density oligonucleotide arrays. These probes measure expression of ~30,000 genes. Expression was then compared between groups [19].	Human
2	56390258	Juvenile Dermatomyositis (JDM)	Skeletal muscle biopsies were gathered from two groups of adolescent females with untreated juvenile dermatomyositis (JDM). The first group consisted of three girls with untreated JDM of <2 months duration. The second group consisted of sixteen girls with untreated JDM of >2 months duration. Total RNA was isolated from the samples and gene expression was assessed using the Affymetrix HG-U133A probe. A Welch t-test was then used to calculate the probabilities of significant gene expression changes between samples [27].	Human
3	26550958	Adult Dermatomyositis (DM)	This data set contains gene data from muscle biopsies gathered from four women with untreated dermatomyositis. A control group of five healthy volunteers were used to assess intergroup differences. Biopsies were analyzed using Affymetrix Human Genome U133A Array. Further information regarding this data set can be found under GEO series accession number GSE5370.	Human
4	127707736	5-azacytidine on mesenchymal progenitors	In this study by Zhou et al., Mesenchymal stem cell lines were obtained from a group of 6–8-week-old ICR mice. The cells were seeded on T-175 flasks and allowed to populate for 24 h. MSC’s were collected when they had grown to 90% confluence. Isolated cells were cultured in 96-well plates at a concentration of 2 × 103 cells/well and treated with differing concentrations of 5-azacytidine, a DNA methylation inhibitor. Treated cells were then removed and assessed for osteogenic gene-expression and calcium mineralization activity [43].	Mus-Musculus
5	60459336	Pyruvate supplementation in myoblast	In this study by Wilson et al., myoblasts from mice were obtained and grown in media with and without pyruvate. This work looked into the relationship between pyruvate supplementation and mitochondrial functioning in vitro. After cells had been treated, they were collected for flow cytometry, confocal microscopy, protein expression studies, gene chip analysis, and more. The results of the gene chip analysis were deposited in the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and are accessible through GEO series accession number GSE5497.	Mus-Musculus
6	46043025	Dystrophin Deficient Cardiomyocyte	In this study by JD porter, a line of dystrophin knockout mice was used to study the functioning of the heart in a Duchene Muscular Dystrophy model. Samples of cardiac myocytes were gathered at 8 weeks and 10.5 months from wild type mice and the dystrophin knockout mice. These samples were then used to analyze gene expression to elucidate intergroup differences. The results of the gene chip analysis were deposited in the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and are accessible through GEO series accession number GSE1471.	Mus-Musculus