Fig. 2. Single-nucleus interrogation of the rat fetal normal and hypoplastic lung identifies four major cell types each with distinct subpopulations.

(A) Schematic of experimental design and in vivo administration of AFSC-EVs in the rat model of CDH. (B) Global Uniform Manifold Approximation and Projection (UMAP) of all nuclei (n = 298,653) included in our study, further delineated by major cell type and subtype. (C) Expression of known cell type–specific markers used to distinguish cellular subtypes within major cell type clusters. Node size is proportional to the percentage of nuclei within the specified cluster, and node color denotes the average expression across nuclei within the specified cluster.