Table 5.
Design and application of whole-cell biosensors for iron monitoring in polar waters.
| Analyte | Sensing Element |
Reporter Gene | Output | Chassis Cell | Field Application |
Drawbacks | References |
|---|---|---|---|---|---|---|---|
| Bioavailable iron | fepA–fes from E. coli |
luxCDABE from V. fischeri |
bioluminescence | P. putida FeLux | Lake Erie; South West Pacific (FeCycle Fe fertilization study) | Sensitivity variations across natural bacterial communities | [34,225] |
| Bioavailable iron |
isiAB promoter from Synechocystis
sp. strain PCC 6803 |
luxAB from V. harveyi |
bioluminescence | Synechococcus sp. strain PCC 7002 | IOW 213, IOW 271, and Bocknis-Eck stations in the Baltic Sea; subarctic Pacific 50 km northeast of Ocean Station Papa (SERIES Fe fertilization study) | Low sensitivity, inadequate representation of picocyanobacteria diversity | [214] |