Figure 2.
Bulk RNA-seq and single-nucleus RNA-sequencing (snRNA-seq) analysis of human adipose tissues
(A) Principal component analysis (PCA) of bulk RNA-seq analyses of human visceral and subcutaneous adipose tissues—initial sample set (hVAT and hSAT, n = 7 and 5 samples, respectively).
(B–E) snRNA-seq analyses of the same hVAT and hSAT as in (A), (64,729 and 37,357 nuclei, respectively, of the initial sample set as in (A)), performed using 10x genomics platform. (B and C) UMAP projection uncovering 15 and 13 uniquely colored annotated clusters of nuclei of hVAT and hSAT, respectively. (D and E) Cell-type annotation of nuclei clusters based on single marker genes, as recently used by Emont M.P.10 in hVAT and hSAT, respectively. ASPC1.APC, adipose stem progenitor cells 1. adipocyte progenitor cells; ASPC2.ASC, adipose stem progenitor cells 2. adipose-derived stem cells; SMC, smooth muscle cells.