Table 2.

Commercially available molecular assays for Pneumocystis jirovecii.

Assay	Manufacturer	Method	Target	Samples
RealStar® Pneumocystis jirovecii	Altona Diagnostics GmbH, Hamburg, Germany	Real-time PCR	mtLSU	Unspecified
PneumoGenius®	PathoNostics B.V., Maastricht, The Netherlands	Multiplex real-time PCR	mtLSU, DHPS mutations	BALF
AusDiagnostics Respiratory panel, pneumonia panel, atypical pneumonia panel	AusDiagnostics Pty Ltd., Mascot, NSW, Australia	Multiplex real-time PCR	Unknown	Swabs, sputum, BALF, tissue, nasopharyngeal aspirate
Bio-Evolution Pneumocystis	Bio-Evolution, Brysur-Marne, France	Real-time PCR	mtLSU	BALF
Pneumocystis ELITe MGB	ELITechGroup S.p.A, Turin, Italy	Quantitative real-time PCR	mtLSU	Bronchial aspirate, sputum
PneumID	OlmDiagnostics, Newcastle, United Kingdom	Real-time PCR	mtLSU	BALF, washings
Fungiplex® Pneumocystis IVD	Bruker Daltonik GmbH, Bremen, Germany	Multiplex real-time PCR	Unknown	BALF, throat swabs
MycoReal® Pneumocystis	Ingenetix GmbH, Vienna, Austria	Real-time PCR	mtLSU	BALF
MycoGENIE® Pneumocystis jirovecii	Ademtech, Pessac, France	Real-time PCR	mtLSU	Respiratory tract samples
AmpliSens® Pneumocystis jirovecii-FRT	Ecoli Dx, s.r.o., Prague, Czechia	Real-time PCR	mtLSU	BALF, sputum, oropharyngeal and tracheal aspirates, lung biopsy, oropharyngeal washes, swabs
RIDA®GENE Pneumocystis jirovecii	R-Biopharm, Darmstadt, Germany	Multiplex Real-time PCR	mtLSU	BALF
Pneumocystis jirovecii (carinii) Real-TM	Sacace, Como, Italy	Real-time PCR	mtLSU	Sputum, BALF, tissue, swabs
LightMix Modular Pneumocystis jiroveci	Roche Diagnostics, Mannheim, Germany	Real-time PCR	MSG	Unspecified
RealCycler PJIR	Progenie-molecular, Valencia, Spain	Real-time PCR	mtLSU	BALF

BALF: bronchoalveolar lavage fluid; DHPS: dihydropteroate synthetase; mtLSU: mitochondrial large subunit; MSG: major surface glycoprotein.