Figure 3.

High CIB1 gene expression in rHGP CRCLM

(A) t-SNE plot: unsupervised Louvain clustering of 2,541 cancer cells with cluster identification. (B) t-SNE plot: clustering of 2,541 cancer cells by HGPs. (C) Volcano plot: differential gene expression analysis of 2,541 single cancer cells of single-cell RNA sequencing (highlighting CIB1). (D) Spatial gene visualization: differential gene expression analysis of three dHGP vs. three rHGP cancer areas of spatial RNA sequencing (highlighting of CIB1).