Table 2.
Identification of fungal isolates with MALDI-TOF MS and the OmniLog ID System. The identification of the moulds was verified by morphological identification [37,38]. Discrepant identification at the genus level is marked in grey.
| MALDI-TOF MS | OmniLog ID System | ||||
|---|---|---|---|---|---|
| Isolate ID | Identification | Score * | Identification | Incubation Time (h) | Similarity Index § |
| 1 | Aspergillus amstelodami | 1.55 | Aspergillus violaceus | 168 | 0.258 |
| 2 | Aspergillus flavus | 1.85 | Aspergillus flavus | 48 | 0.583 |
| 3 | Aspergillus fumigatus | 2.02 | Aspergillus fumigatus | 48 | 0.711 |
| 4 | Aspergillus fumigatus | 1.89 | Aspergillus fumigatus | 72 | 0.652 |
| 5 | Aspergillus glaucus | 2.20 | Aspergillus rugulosus | 96 | 0.316 |
| 6 | Cladosporium cladosporioides | 1.50 | Cladosporium sphaerospermum | 96 | 0.285 |
| 7 | Cladosporium sp. | 1.71 | Cladosporium tenuissimum | 96 | 0.566 |
| 8 | Cutaneotrichosporon dermatis | 2.20 | Hannaella luteola | 48 | 0.832 |
| 9 | Cutaneotrichosporon dermatis | 2.28 | Bullera alba | 48 | 0.868 |
| 10 | Meyerozyma guilliermondii | 2.18 | Meyerozyma guilliermondii | 48 | 0.744 |
| 11 | Meyerozyma guilliermondii | 2.34 | Meyerozyma guilliermondii | 48 | 0.699 |
| 12 | Penicillium brevicompactum | 1.90 | Penicillium freii | 48 | 0.53 |
| 13 | Penicillium brevicompactum | 2.07 | Penicillium chrysogenum | 168 | 0.535 |
| 14 | Penicillium brevicompactum | 2.24 | Penicillium freii | 48 | 0.423 |
| 15 | Penicillium brevicompactum | 1.95 | Penicillium viridictum | 96 | 0.575 |
| 16 | Penicillium chrysogenum | 1.47 | NA | NA | NA |
| 17 | Penicillium chrysogenum | 1.39 | NA | NA | NA |
| 18 | Penicillium chrysogenum | 1.68 | Penicillium chrysogenum | 96 | 0.535 |
| 19 | Penicillium chrysogenum | 1.49 | NA | NA | NA |
| 20 | Penicillium chrysogenum | 1.48 | NA | NA | NA |
| 21 | Penicillium chrysogenum | 1.67 | NA | NA | NA |
| 22 | Penicillium chrysogenum | 1.84 | Penicillium thomii | 168 | 0.425 |
| 23 | Penicillium chrysogenum | 1.93 | Penicillium chrysogenum | 48 | 0.671 |
| 24 | Penicillium chrysogenum | 1.78 | Penicillium chrysogenum | 168 | 0.552 |
| 25 | Penicillium chrysogenum | 1.89 | Penicillium freii | 48 | 0.438 |
| 26 | Penicillium chrysogenum | 1.93 | NA | NA | NA |
| 27 | Penicillium commune | 2.45 | Penicillium commune | 96 | 0.526 |
| 28 | Penicillium commune | 1.86 | Penicillium cyclopium | 168 | 0.22 |
| 29 | Penicillium digitatum | 1.75 | Penicillium javanicum | 96 | 0.543 |
| 30 | Penicillium digitatum | 2.27 | Penicillium tricolor | 72 | 0.425 |
| 31 | Penicillium italicum | 1.74 | Penicillium freii | 48 | 0.585 |
| 32 | Penicillium italicum | 1.53 | Penicillium hirsutum | 168 | 0.321 |
| 33 | Penicillium italicum | 1.80 | Penicillium commune | 48 | 0.293 |
| 34 | Penicillium italicum | 2.82 | Penicillium hirsutum | 96 | 0.323 |
| 35 | Penicillium sp. | 1.72 | Penicillium citrinum | 168 | 0.411 |
| 36 | Penicillium sp. | 1.48 | Penicillium citrinum | 48 | 0.430 |
NA—not applicable (subcultivations not successful). * A MALDI score of >2.0 indicates a reliable identification at the species level; a MALDI score between 1.70 and 1.99 indicates a reliable identification at the genus level. § A similarity index of ≥0.7 after 48 h, ≥0.65 after 72 h, or ≥0.6 after 96 h of incubation in combination with the morphological characteristics indicates identification to species level.