Table 3.
Molecular identification of discrepant MALDI-TOF MS and OmniLog ID System identifications related to results of the 16S rRNA gene sequencing. Successful molecular species-level identifications are marked in grey.
| MALDI-TOF MS | OmniLog ID System | 16S rRNA Sequencing | |||||
|---|---|---|---|---|---|---|---|
| Isolate ID | Identification | Score * | Identification | Probability (%) | Identification | Probability (%) § | Equally Possible Molecular Identifications |
| 6 | Aerococcus viridans | 2.23 | Streptococcus orisratti | 89.9 | Aerococcus sp. | 98.9 | A. viridans/A. sanguinicola/A. suis |
| 7 | Bacillus mycoides | 1.95 | Paenibacillus anaericanus | 70.4 | Bacillus sp. | 99.7 | B. wiedmannii/B. tropicus/B. proteolyticus |
| 11 | Enterococcus moraviensis | 1.83 | Enterococcus gallinarum | 85.2 | Enterococcus sp. | 100.0 | E. moraviensis/E. wangshanyuanii/E. ureilyticus |
| 12 | Exiguobacterium sp. | 2.19 | Bacillus halodurans | 95.2 | Exiguobacterium sp. | 100.0 | E. gingdaonense/E. algae/E. arabatum |
| 16 | Microbacterium maritypicum | 1.61 | Microbacterium liquefaciens | 61.0 | Microbacterium sp. | 99.7 | M. luteolum/M. algeriense/M. saperdae |
| 17 | Microbacterium oxydans | 2.39 | Microbacterium maritypicum | 94.8 | Microbacterium sp. | 98.4 | M. luteolum/M. algeriense/M. saperdae |
| 19 | Microbacterium phllosophaerae | 1.93 | Microbacterium maritypicum | 88.7 | Microbacterium sp. | 99.7 | M. luteolum/M. algeriense/M. saperdae |
| 18 | Microbacterium phllosophaerae | 2.01 | Microbacterium maritypicum | 82.9 | Microbacterium sp. | 99.7 | M. luteolum/M. algeriense/M. saperdae |
| 20 | Microbacterium phllosophaerae | 1.68 | Microbacterium maritypicum | 95.0 | Microbacterium sp. | 99.3 | M. luteolum/M. algeriense/M. saperdae |
| 40 | Micrococcus luteus | 2.12 | Janibacter anophelis/hoylei | 85.3 | Micrococcus sp. | 99.4 | M. luteus/M. antarcticus/M. aloeverae |
| 39 | Micrococcus luteus | 2.20 | Bacillus krulwichiae | N | Micrococcus sp. | 100.0 | M. luteus/M. antarcticus/M. aloeverae |
| 33 | Micrococcus luteus | 2.27 | Microbacterium maritypicum | 82.9 | Micrococcus sp. | 100.0 | M. luteus/M. antarcticus/M. aloeverae |
| 35 | Micrococcus luteus | 2.40 | Brevibacterium otitidis | 74.1 | Micrococcus sp. | 99.7 | M. luteus/M. antarcticus/M. aloeverae |
| 38 | Micrococcus luteus | 2.40 | Brevibacterium otitidis | 92.0 | Micrococcus sp. | 100.0 | M. luteus/M. antarcticus/M. aloeverae |
| 44 | Pseudarthrobacter oxydans | 2.39 | Janibacter anophelis/hoylei | 87.8 | Pseudarthrobacter sp. | 99.2 | P. psychrotolerans/P. phenanthrenivorans/P. nitrophenolicus |
| 45 | Pseudoclavibacter helveolus | 2.30 | Virgibacillus salexigens | 86.5 | Paenibacillus purispatii | 100.0 | Paenibacillus purispatii |
| 48 | Pseudomonas putida | 1.42 | Brachybacterium muris | 87.5 | Macrococcus sp. | 100.0 | M. canis/M. caseolyticus/M. equipercicus |
| 49 | Pseudomonas putida | 1.45 | Serpens flexibilis | 86.8 | Pseudomonas sp. | 99.8 | Pseudomonas sp. |
| 68 | Staphylococcus hominis | 2.27 | Paenibacillus provencensis | 87.0 | Staphylococcus sp. | 100.0 | S. pragensis/S. borealis/S. croceilyticus |
| 80 | Staphylococcus warneri | 2.00 | Staphylococcus aureus | 96.0 | Staphylococcus warneri | 100.0 | Staphylococcus warneri |
| 85 | Streptomyces violaceoruber | 1.38 | Paenibacillus sanguinis | 88.1 | Pseudoclavibacter terrae | 100.0 | Pseudoclavibacter terrae |
N—identification probability not available. * A MALDI score of >2.0 indicates a reliable identification at the species level; a MALDI score between 1.70 and 1.99 indicates a reliable identification at the genus level. § A match of ≥99.0% or ≥97.0% is considered to belong to the same species or genus, respectively.