Figure 7.

Complex I forward and reverse electron transfer emissions in tibialis anterior and diaphragm muscle of epithelial ovarian cancer (EOC) injected mice. Complex I forward electron transfer (FET) and complex I reverse electron transfer (RET) is schematically depicted (A & B). In FET mitochondrial H₂O₂ emission was supported by pyruvate (10 mM) and malate (2 mM) to generate maximal rates and with ADP to assess H₂O₂ emission during OXPHOS. This experiment was repeated to assess RET H₂O₂ emission by using succinate (10 mM) as opposed to pyruvate and malate. FET and RET H₂O₂ emissions were assessed in the TA of EOC injected mice and a summary of changes compared to control is depicted (C–G). This was repeated in the diaphragm (H–L). Results represent mean ± SD. Lettering denotes statistical significance when different from each other (p < 0.05). β p < 0.05 Control versus 75 Day; λ p < 0.05 75 Day vs 90 Day; δ p < 0.05 Control versus 90 Day. A one-way ANOVA or Kruskal–Wallis test was used when data did not fit normality in figure C, E, H and J. A two-way ANOVA was used in figured D, F, I and K. All ANOVAs were followed by a two-stage step-up method of Benjamini, Krieger and Yukutieli multiple comparisons test. Oxidative phosphorylation (OXPHOS); manganese superoxide dismutase (MnSOD); electron (e−); superoxide (O₂⁻). C57BL/6J female mice ∼75 days post PBS injection as controls (CTRL); C57BL/6J female mice ∼45 days post ovarian cancer injection (45 Days); C57BL/6J female mice ∼75 days post ovarian cancer injection (75 Days); C57BL/6J female mice ∼90 days post ovarian cancer injection (90 Days).