Fig. 2.

Study protocol on days 1–3. At 16 h into the 24 h fast (t = 0 min) participants recorded their baseline Visual Analogue Scale (VAS)-based appetite assessments and then consumed a delayed digestion capsule containing either a placebo, a low-dose Amarasate® hop extract or a high-dose Amarasate hop extract. Every 30 min, VAS-based appetite assessment questionnaires were taken from 16 h until 24 h into the 24 h fast. Then at 20 h into the 24 h fast (t = 240 min) a second treatment capsule matched to the first was given.