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. 2023 Dec 12;326(1):L111–L123. doi: 10.1152/ajplung.00155.2023

Figure 1.

Figure 1.

Transforming growth factor-β (TGF-β)-induced expression of anoctamin 1 (ANO1) in human lung fibroblasts. Human lung fibroblasts were serum starved in 0.1% FBS for 48 h, followed by treatment with TGF-β (1 ng/mL) for 24 or 48 h, as indicated. A: ANO1 mRNA expression after 24-h treatment with TGF-β as determined by quantitative RT-PCR. Data are mean values ± SD. ***P < 0.001, t test. B: Western blot analysis and quantitation of ANO1 protein expression in response TGF-β. Data are mean values normalized to control cells ± SD. *P < 0.05, ***P < 0.001, Kruskal-Wallis test with Dunn’s correction for multiple comparisons. C: effect of the TGF-β receptor II (TGFBRII) inhibitor SB-431542 (5 µM, 30-min preincubation) on TGF-β-induced ANO1 protein. expression (48 h). Shown are representative images of ANO1 immunoreactivity and quantification. Data are mean immunoreactivity values normalized to TGF-β-treated cells ± SD. ***P < 0.001, one-population t test with Bonferroni correction for multiple comparisons.