Fig. 5.

ASFVGZΔA137R produces a reduced ratio of infectious virus titer-to-genome copies in MA104 ​cells. A Genome copies of progeny ASFVGZΔA137R or ASFVGZ. MA104 cells in 24-well plates were infected with either ASFVGZΔA137R or ASFVGZ at genome copies of 10⁸ or 10⁹. The genome copies in supernatant were determined by qPCR at 24 hpi. B Infectious progeny virus production of ASFVGZΔA137R or ASFVGZ. The titers of infectious progeny virions from group A were detected according to the Spearman–Kärber method. C ASFVGZΔA137R or ASFVGZ infectious virus titer-to-genome copies ratios. The infectious virus titer-to-genome copies ratios were calculated based on the ratio of (B) to the (A) at 24 hpi (n ​= ​3). The significance of differences between groups was determined using Student's t-test (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001).