Fig. 4.

The characteristic of specific memory B and T cell were elicited by various immunization strategies in mice. A Frequencies of specific memory B cells in mice were analyzed by flow cytometry in mice immunized with various immunization strategies. B Memory phenotypic profiles of the specific MERS-CoV-S CD8⁺ T cells elicited in mice immunized with various immunization strategies. Percentages of central memory (Tcm) (CD44^high and CD62L^high) and T effector memory (Tem) (CD44^high and CD62L^low) CD8⁺ T cells specific for MERS-S peptide pool, and expressing any of the markers, CD107a, IFN-g, TNF-a. C MERS-S specific polyfunctional CD8⁺ Tem cell responses were detected by ICS. Result analysis referenced Fig. 3C. Bars represented the mean ​± ​SD; results were analyzed by Kruskal-Wallis test. Blank group n ​= ​5; VTT, vaccinated groups n ​= ​6.