FIG. 3.

HIV-1 Gag peptide-specific cytotoxicity of chimpanzee PBMC is mediated by CD8⁺ T cells. PBMC from chimpanzees C-420, C-424, and C-487 were stimulated with peptides 8, 17, and 5, respectively. PBMC from chimpanzee C-1196 were stimulated with peptides 8 (white bars), 9 (hatched bars), and 20 (black bars). These effector cells were separated into CD8⁺-lymphocyte-depleted or -enriched populations by using CD8-specific immunomagnetic beads and then used in a ⁵¹Cr release cytotoxicity assay with peptide-pulsed autologous B-LCL targets at an effector/target ratio of 20:1. PBL, peripheral blood lymphocytes.