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eIF4G cleavage in poliovirus-infected cells. Cytoplasmic extracts from mock-infected (lanes c) and poliovirus-infected (lanes i) cells were prepared at the times indicated as described in the legend to Fig. 1. Aliquots of the cytoplasmic extracts were precipitated with acetone for protein analysis by SDS-PAGE and Western blotting using the anti-eIF4G antibody as previously described (18).
