Figure 10.

Performance characterization of SelDeg51 after spray drying. (b) Western blot analysis of SelDeg51-mediated FKBP51 degradation by neat SelDeg51 as well as the SDD with 30% drug load after 24 h of treatment in HEK293T cells. GAPDGH was determined as loading control. (a) Binding to the E3 ligase VHL as determined by fluorescence polarization of neat SelDeg51, the SDD, a physical mixture with 30% drug load and neat PVA in presence of a saturating excess of FKBP51^FK1HP: high protein control representing fully bound VHL tracer; DMSO with no additional substance treatment; No VCB: Assay mix without VHL/EloB/EloC, representing fully unbound tracer.