Figure 3.
Effects of zileuton on cell proliferation, migration, and intracellular signaling. (A) The change in LTD4 concentration in the medium supernatant caused by zileuton administration was measured by a CysLTR1 ELISA kit. Data represent the means of four independent samples. Data are presented as mean ± standard deviation. * p < 0.05, ** p < 0.01, and *** p < 0.001. (B) Graphs of relative cell numbers compared with the control at 72 h quantified by a Cell Counting Kit-8 assay in high-CYSLTR1-expressing cell lines (RBE and SSP-25). Cells were treated with zileuton at 0–150 µmol/L. (C) Representative images obtained at 12 or 48 h after a scratch wound was made in confluent monolayers of RBE and SSP-25 cells. After the scratch, 50 µmol/L of zileuton was added. The migration rates were quantified by measuring the area of the injured region. (D) Western blotting for RBE and SSP-25 treated with zileuton at 100 µmol/L for 24 h. P-AKT levels were normalized against AKT, and p-ERK1/2 levels were normalized against ERK1/2. These represented the means of three independent experiments. Bars indicate standard deviation.