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. 1999 Aug;73(8):7087–7092. doi: 10.1128/jvi.73.8.7087-7092.1999

FIG. 2.

FIG. 2

HIV-1 replication is inhibited in lymphocytes transduced by HIV-derived vectors. The inhibitory activity is mediated by the vector LTR. (A) SupT1 cells transduced by HIV-derived (□), ΔU3 HIV-derived (Inline graphic), and MLV-derived (Inline graphic) vectors encoding EGFP or nontransduced cells (■) were infected with the indicated p24 equivalent of HIV-1 and scored for the accumulation of p24 antigen in the culture supernatant (upper panels) and cell viability (lower panels). (B) SupT1 cells were transfected (grey markers, with each marker representing an individual clone) or transduced (□) with HIV-derived vector encoding the neomycin resistance gene, selected in G418-containing medium, and infected with HIV-1 as above; control cells are also shown (■). HIV-1 replication was inhibited in all the cultures containing the vector, independent of the mode of delivery. (C) Primary CD4-positive lymphocytes were transduced with HIV-derived (□) and ΔU3 HIV-derived (Inline graphic) vectors encoding EGFP and selected for transgene expression or were not transduced and mock sorted (■) and infected as described above. The averages of duplicate determinations are shown for a representative experiment of three performed.