Figure 3.

Analysis of ROS production in B16-F10 cells after treatments. (A) Representative histogram of reactive oxygen species production by B16-F10 cells pre- and post-treatments. (B) Quantification of the percentage of cells in each treatment group after 2 h. Cells were treated with NAC (negative control), H₂O₂ (positive control), photodynamic therapy (PDT), and mitoxantrone (MTX). The results presented are from three independent experiments, with the average percentage of cells in each treatment group ± SEM. * p < 0.05 compared with control.