Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 14;16(7):941. doi: 10.3390/pharmaceutics16070941

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunostaining of HMGB1 and quantitative fluorescence analysis in B16-F10 cells after 24 h of treatment. (A) DAPI staining the nucleus in blue, green marking the HMGB1 protein, and the overlay of both labels. Arrows indicate the extravasation of HMGB1 from the nucleus and its presence in the cellular cytoplasm. The 3D reconstruction videos can be found in the Supplementary Material (Video S1, Video S2, and Video S3). (B–D) Co-localization plots of the 3D reconstructions, where the blue color represents voxels positive for the nucleus (DAPI), and the green color represents voxels positive for HMGB1. Points present in the intermediate region of the plot represent co-localized voxels. The quantification of co-localization is depicted in (E).