FIG. 5.

RNA replication in CHO cells transfected with pIND-FHV1[0,0]. Cells were transfected with plasmid pIND-FHV1[0,0] and incubated at 37°C for 24 h. The cells were shifted to 28°C and treated with muristerone A for 24 h. Thereafter, hormone was replenished daily in one set of dishes (□) and removed from a parallel set by washing and replacing with complete medium that lacked the hormone (○). At daily intervals, cells were pulse-labeled with [³H]uridine in the presence of actinomycin D. Labeled RNAs were isolated and quantitated as described for Fig. 1. The rate of RNA replication (in counts per minute per 1.7 × 10⁵ cells) was expressed as a function of time after hormone addition.