Table 1.

Defined and complex medium composition for the biosynthesis of γ-PGA by Bacillus subtilis natto.

Medium Name	Components	Quantity (g/L)
GS	Sucrose	50
	L-Glutamate	20
	NaCl	50
	KH2PO4	2.7
	Na2HPO4	4.2
	MgSO4·7H2O	5
	Murashige–Skoog vitamin solution	1 mL/L
GS25	Sucrose	50
	L-Glutamate	20
	NaCl	25
	KH2PO4	2.7
	Na2HPO4	4.2
	MgSO4·7H2O	5
	Murashige–Skoog vitamin solution	1 mL/L
GS0	Sucrose	50
	L-Glutamate	20
	NaCl	0
	KH2PO4	2.7
	Na2HPO4	4.2
	MgSO4·7H2O	5
	Murashige–Skoog vitamin solution	1 mL/L
Ap0	Ascophyllum nodosum	40
	Sucrose	25
	L-Glutamate	10
	NaCl	0
Ap25	Ascophyllum nodosum	40
	Sucrose	25
	L-Glutamate	10
	NaCl	25
Ap50	Ascophyllum nodosum	40
	Sucrose	25
	L-Glutamate	10
	NaCl	50
ApMn0	Ascophyllum nodosum	40
	Sucrose	25
	L-glutamate	10
	NaCl	0
	MnSO4	0.415 g/L
ApMn25	Ascophyllum nodosum	40
	Sucrose	25
	L-Glutamate	10
	NaCl	25
	MnSO4	0.415 g/L
ApMn50	Ascophyllum nodosum	40
	Sucrose	25
	L-Glutamate	10
	NaCl	50
	MnSO4	0.415 g/L