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. 2024 Jul 9;12(7):755. doi: 10.3390/vaccines12070755

Figure 2.

Figure 2

Optimizing YF-ZIK production in selected host cells. Effect of MOI and temperature on infectious virus titers in ambr15 system. (a) MOI screening. Cells infected at MOI 0.1 (blue squares), 0.01 (red triangles), 0.001 (green diamonds), and 10−4 (purple circles). Values from a single cultivation. (b) Impact of temperature on maximum viable cell concentrations (VCCmax) post infection (left) and resulting infectious virus titers (right). Values mean ± STD of n = 2 for runs at 37 °C and n = 4 for runs at 34 °C. BHKPEM and HEKPEM at 34 °C as single runs. Two-way ANOVA followed by Šidák’s multiple comparison; p values * < 0.05 were considered significant. (c) Optimized productions were repeated as a biological triplicate to confirm reproducibility and robustness. VCCs (full symbols) and culture viabilities (empty symbols and dashed lines) are shown left, infectious virus titers right. Dashed lines indicate time of infection. Values are represented as the mean ± STD of n = 3.