Table 1.
Growth characteristics of cell lines before and after infection and virus yields.
| Cell Line | VCCmax p.i. (106 Cells/mL) |
µ (1/h) | µinf (1/h) | inf. vir. Titer (106 PFU/mL) |
CSVY (PFU/Cell) |
|---|---|---|---|---|---|
| VeroPDM | 4.0 ± 0.4 | 0.02 ± 0.003 | 0.015 | <0.01 | <0.1 |
| VeroPDM/MDXK | 5.6 ± 0.4 | 0.022 | 0.014 | <0.01 | <0.1 |
| pIX | 14.1 ± 0.1 | 0.024 | 0.024 | 55.9 ± 29.1 | 4 ± 2 |
| CR | 14.8 ± 0.9 | 0.026 ± 0.002 | 0.021 ± 0.001 | 6.0 ± 2.7 | <1 |
| CCX.E10 | 5.7 ± 0.1 | 0.020 | 0.010 | 0.8 ± 0.1 | <0.1 |
| BHKPEM | 10.0 ± 0.3 | 0.024 ± 0.002 | 0.017 ± 0.001 | <0.01 | <0.1 |
| BHKHIP | 12.4 ± 0.1 | 0.027 | 0.018 | 0.02 | <0.1 |
| BHK-PPEM | 4.8 ± 0.3 | 0.024 ± 0.001 | 0.022 ± 0.001 | <0.01 | <0.1 |
| HEKPEM | 6.2 ± 0.4 | 0.021 ± 0.002 | 0.020 ± 0.001 | 1.1 ± 0.4 | <1 |
| HEKFS | 2.6 ± 0.7 | 0.008 | 0.010 ± 0.002 | 10.6 ± 5.8 | 4 ± 2 |
| HEKDyn | 6.1 ± 0.3 | 0.018 | 0.014 | 55.0 ± 13.7 | 9 ± 2 |
| HEKvs | 6.8 ± 0.4 | 0.017 ± 0.002 | 0.017 ± 0.002 | 0.3 ± 0.1 | <0.1 |
| PBG.PK-21 | 7.7 ± 0.5 | 0.014 ± 0.001 | 0.011 | 0.1 | <0.1 |
| MDCKMDXK | 16.2 ± 0.3 | 0.035 ± 0.001 | 0.028 | <0.01 | <0.1 |
| MDCKDM | 8.1 ± 0.1 | 0.02 ± 0.002 | 0.017 | <0.01 | <0.1 |
| MDCK4C | 8.4 ± 0.3 | 0.023 ± 0.001 | 0.013 | <0.01 | <0.1 |
| MDCKS | 16.3 ± 0.2 | 0.031 ± 0.002 | 0.027 | <0.01 | <0.1 |
| Veroadh | 2.5 ± 0.1 | 0.028 ± 0.001 | 0.003 ± 0.001 | 1.5 ± 0.1 | <1 |
Maximum viable cell concentration (VCCmax) post infection (p.i.); cell-specific growth rate before (µ) and after infection (µinf); maximum infectious virus titer (inf. vir. titer); cell-specific virus yield (CSVY). Orange: high producers; white: low producers; gray: no producers. All runs carried out in duplicates. Values mean ± STD. If no deviation is given, values are too small to be displayed.