Table 2.
Growth and productivity of selected cell lines under optimized conditions.
| Cell Line | MOI | VCCmax p.i. (106 Cells/mL) |
µ (1/h) |
µinf (1/h) |
inf. vir. Titer (107 PFU/mL) | CSVY (PFU/Cell) |
|---|---|---|---|---|---|---|
| pIX | 0.01 | 12.3 ± 0.2 | 0.022 ± 0.006 | 0.016 ± 0.006 | 7.7 ± 2.3 | 6 ± 2 |
| CR | 0.001 | 14.3 ± 0.6 | 0.021 ± 0.006 | 0.015 ± 0.004 | 8.5 ± 1.1 | 6 ± 1 |
| HEKDyn | 0.01 | 6.0 ± 0.6 | 0.019 ± 0.004 | 0.013 ± 0.008 | 11.3 ± 1.2 | 19 ± 2 |
| HEKFS | 0.001 | 3.5 ± 0.2 | 0.028 ± 0.007 | 0.012 ± 0.007 | 8.3 ± 3.6 | 24 ± 11 |
Maximum viable cell concentration (VCCmax) post infection (p.i.); growth rate before (µ) and after infection (µinf); maximum infectious virus titer (inf. vir. titer); cell-specific virus yield (CSVY). Orange, high producers; white, low producers; gray, no producers. All runs carried out in duplicates. Values are given as the mean ± STD with n = 3.