Figure 5.

Maximum Likelihood tree of DENV-2 based on envelope sequences (n = 2375). DENV-2 samples were collected from 16 countries across three regions of Asia, with a maximum number of 20 sequences per year. The nucleotide sequences were aligned using MAFFT v.7 and a phylogenetic tree was constructed in IQ-TREE v.2.2.2.6 using the GTR + F + I + R6 model with 1000 ultrafast bootstrap replicates. Bootstrap values are shown at the tree branches. Each terminal node is colored according to the country of origin. Locations, genotypes, and sampling years are shown in the legends. The scale represents the substitution rate per site. The panel on the right shows the genotypes and range of sampling years corresponding to each taxon. Lineages are indicated adjacent to the key branches, and clades and subclades in parentheses are indicated adjacent to the key clusters, with colors corresponding to each genotype.