Skip to main content
. 2024 Jul 11;16(7):311. doi: 10.3390/toxins16070311

Figure 5.

Figure 5

Effects of the A1-domain of LT-IIc on cytotoxicity and binding affinity. (A) Cartoon representation of the A1-domain (light blue), A2-domain (yellow), and B-pentamer (blue) of LT-IIc holotoxin and LT-IIc-A2-Flg chimeric protein in which the A1-domain is replaced by a Flag epitope tag (orange). (B) MDA-MB-231 cells were treated with 62 nM LT-IIc, LT-IIc-A2-Flg, or LT-IIc-B5 for 48 h and analyzed for viability compared to untreated cells measured by MTT assay (n = 6). Data points represent mean +/− SEM. Statistical significance was measured by one-way ANOVA. *** p < 0.0001. (C) Example of one of the four flow cytometric analyses measuring the effect of unlabeled LT-IIc (light blue) and unlabeled LT-IIc-A2-Flg (orange) on the mean fluorescent intensity of MDA-MB-231 cells stained with Alexa Fluor 488-labeled LT-IIc (red).