Figure 2.

(A) Experimental design to acclimatize hamsters to WBP chambers and to animal holders for mechanical restraint. Medetomidine injection was employed as chemical restraint and did not require additional acclimatization other than to the WBP chambers. All animals were allowed to acclimatize to the WBP chambers for increasing time intervals. (B) Absolute bf values with ranges on study day—14. Note the low bf values of chemically restrained hamsters compared to unrestrained hamsters. Thin lines represent individual animals. (C) Representative photographs taken during WBP measurements. (D) Wet fur of mechanically restrained hamsters due to condensation of exhalations in the animal holder.