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Inhibition of translation from capped 5′ UTRs by 7-methyl GTP. Capped transcripts of p77N and p210N were made as described in the text and translated in wheat germ extract in the presence of radiolabeled methionine, and the products were analyzed by SDS-PAGE. Lane 1, molecular weight markers; lanes 2 and 4, no 7-methyl GTP; lanes 3 and 5, 0.2 mM 7-methyl GTP.
